Existence of Multi Drug Resistance Pathogen in Soil of Hospital Disposal Site

Abstract

Hospital waste is a potential health hazards to the health care workers and public acquired infection, transfusion transmitted diseases. Increasing land and water pollution lead to increasing possibility of contracting many diseases. Study has compelled the authorities to think seriously about hospital wastes and the disease transmitted through their improper disposal. Soil samples were collected from hospital in Jaipur city. A total of six isolated were identified and purified from the samples, further screened for individual antibiotics at their respective concentrations and all the six isolates were found to be strong resistant against antibiotics selected in the study. In this present study, Pseudomonas sp only possessed a positive virulence characteristic such as hemolysis, protease, coagulase, lecithinase, pyocyanin and lipase when compare to other species like E. coli, Proteus, Bacillus, Klebsiella, Staphylococcus sp. These are all very low in prevalence of enzymes associated with bacterial pathogens. The total of 11 antibiotics were used included; Tetracycline, Ampicillin, Sparfloxacin, Co-timoxazole, Gatifloxacin, Cefrizoxime, Amoxicillin, Ciprofloxacin, Nitrofurantoin, Ofloxacin and Streptomycin. All strains were found resistant to Tetracycline, Ampicillin Sparfloxacin, Co-timoxazole and Gatifloxacin. 8 antibiotics showed an intermediate resistance. All isolates were found sensitive to Nitrofurantoin, Ofloxacin and Streptomycin. The aim of the study is isolation, identification and antibiotic profiling of microbes from soil contaminated with hospital waste dumping..